Phytochemical Analysis of Cleome viscosa Active Polyphenolic Compounds Possessing Antidiabetic Activity

Pharmacognosy Research,2022,14,2,195-203.DOI:10.5530/pres.14.2.28Published:April 2022Type:Original ArticleAuthors:Suresh Yarrappagaari, Lavanya Thopireddy, Srinivasulu Cheemanapalli, Venkata Rami Reddy Narala, Kakarla Chandra Mohan, and Rajeswara Saddala Author(s) affiliations:Suresh Yarrappagaari1, Thopireddy2, Cheemanapalli3, Narala4, Mohan2, Saddala1,* 1Division of Ethnopharmacology, Department Biotechnology, School Herbal Studies Naturo Sciences, Dravidian University, Kuppam, Andhra Pradesh, INDIA. 2Department Zoology, Kasireddy Venkatareddy Government College for Women (A), Kurnool, 3Regional Ayurveda Research Institute, Itanagar, Arumachal 4Department Animal Sciences (Zoology), Yogi Vemana Kadapa, Abstract:Background: The counteractive action diabetes by plant polyphenols along with their utilization in the treatment diabetic difficulties is expanding significance from epidemiological information vitro vivo examinations. Objectives: We assessed antihyperglycemic impacts methanolic (MeCV), aqueous (AqCV), ethyl acetate (EaCV) n-hexane (NhCV) extracts Cleome viscosa whole plant. Materials Methods: Qualitative phytochemical analysis antidiabetic activities glucose diffusion, inhibitory movement starch processing enzymes i.e. α-glucosidase α-amylase, uptake yeast cell method also non-enzymatic (glycosylation Hb). identification bioactive was categorized using HPLC/UV. Results: To evaluate comparative power these extracts, concentrations required 50% 100% inhibition enzyme were resolved. Greatest utilized to an inhibitor’s relative viability. Results demonstrated that MeCV strongly diffused inhibited both α-amylase great impact on cells glycosylation Hb equivalent intensity respectively than acarbose. While NhCV showed less α-glucosidase, Hb. Gallic acid (2.45%), protocatechuic hexoside (0.83%), chlorogenic (0.77%), catechin (0.53%), quercetin (0.80%), p-hydroxybenzoic (0.78%), p-coumaric (0.07%) main compounds MeCV. Conclusion: Based our results we concluded firmly enzymatic due rich content present Key words: Enzyme inhibition, α-Glucosidase, α-Amylase, viscosa, HPLC/UV, View:PDF (776.2 KB)